Goat anti-Rabbit IgG H+L Highly Cross-Adsorbed, Alexa Fluor™
₫ 182200
a11034 Print Goat anti-Rabbit IgG H+L Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, Invitrogen™.
Western blot analysis of various lysates using BRCA1 Rabbit pAb A11034 at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG H+L AS014 at 1:10000 dilution. Lysates/proteins: 25μg per lane. a a11034.com Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit RM00021. Exposure time: 60s
Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. a a11034.com In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488
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